LOCUS       sequence-66459-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Han C, Jan LY, Jan YN
  TITLE     Enhancer-driven membrane markers for analysis of nonautonomous 
            mechanisms reveal neuron-glia interactions in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
            10.1073/pnas.1106386108. Epub 2011 May 23.
  PUBMED    21606367
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1106386108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-7- 7"; volume: "108"; issue: "23"; pages: "9673-8"
COMMENT     Sequence Label: pDEST-HemmarG
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             373..678
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     557..576
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(719..843)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag
       61 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
      121 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
      181 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
      241 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
      301 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
      361 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
      421 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
      481 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc
      541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
      601 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
      661 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      721 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      781 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      841 ggtgatgatc tggtctagcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
      901 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag c
//
