LOCUS       sequence-66463-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: FU-tetO-Gateway
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..271)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(302..321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(303..321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(400..424)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(421..459)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(492..510,511..533,534..552,553..575,576..594,595..617,
                     618..636,637..659,660..678,679..701,702..720,721..743,
                     744..762)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc agcttttttg tacaaacttg ttgatcaagc ttacctagcc agcttgggtc
      301 tccctatagt gagtcgtatt acaattcggg gccgcggagg ctggatcggt cccggtgtct
      361 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
      421 agctctgctt atataggcct cccaccgtac acgcctacct cgacccgggt accgagctcg
      481 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      541 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      601 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      661 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
      721 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgataacc
      781 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gta
//
