LOCUS       sequence-66468-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..32
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(81..114)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(123..156)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      165..174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggatatcaca agtttgtaca aaaaagcagg ctttaaagga acgcacgtaa ctataacggt
       61 cctaaggtag cgaacctagg ataacttcgt atagcataca ttatacgaag ttatcggcgc
      121 gcataacttc gtataggata ctttatacga agttatcaag cgctgccacc atggtgagca
      181 agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcgcatgg
      241 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
      301 agggctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
      361 acatcctgtc ccctcatttc acctacggct ccaaggccta cgtgaagcac cccgccgaca
      421 tccccgacta cttcaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
      481 acgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
      541 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg atgcagaaga
      601 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggt gccctgaagg
      661 gcaagatcaa gatgaggctg aagctgaagg acggcggcca ctacacctcc gaggtcaaga
      721 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt
      781 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
      841 gccgccactc cac
//
