LOCUS       sequence-66469-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.0
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    151..175
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(224..257)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(266..299)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      308..317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagaggggct gaggtattca tcatgtgctc cgtggatctc aagccctcaa ggtaaatggg
       61 gacccacctg tcctaccagc tggctgacct gtagctttcc ccaccacaga atccaagtcg
      121 gaactcttgg cacctagagg atctcgaatc acaagtttgt acaaaaaagc aggctttaaa
      181 ggaacgcacg taactataac ggtcctaagg tagcgaacct aggataactt cgtatagcat
      241 acattatacg aagttatcgg cgcgcataac ttcgtatagg atactttata cgaagttatc
      301 aagcgctgcc accatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt
      361 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      421 cgagggcgag ggccgcccct acgagggctt tcagaccgct aagctgaagg tgaccaaggg
      481 tggccccctg cccttcgcct gggacatcct gtcccctcat ttcacctacg gctccaaggc
      541 ctacgtgaag caccccgccg acatccccga ctacttcaag ctgtccttcc ccgagggctt
      601 caagtgggag cgcgtgatga actacgagga cggcggcgtg gtgaccgtga cccaggactc
      661 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      721 cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      781 ccccgaggac ggtgccctga agggcaagat caagatgagg ctgaagctga
//
