LOCUS       sequence-66470-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(185..218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(227..260)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(269..302)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      311..320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             323..343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agagaggggc tgaggtattc atcatgtgct ccgtggatct caagccctca aggtaaatgg
       61 ggacccacct gtcctaccag ctggctgacc tgtagctttc cccaccacag aatccaagtc
      121 ggaactcttg gcacctagag gatctcgagc gtaactataa cggtcctaag gtagcgaacc
      181 taggataact tcgtatagca tacattatac gaagttatcg gcgcgcataa cttcgtatag
      241 gatactttat acgaagttat caagcgctat aacttcgtat agtatacctt atacgaagtt
      301 atctgatatc gccaccatgg ctcctaagaa gaagaggaag gtgatgagca gcggcgccct
      361 gctgttccac ggcaagatcc cctacgtggt ggagatggag ggcaatgtgg atggccacac
      421 cttcagcatc cgcggcaagg gctacggcga tgccagcgtg ggcaaggtgg atgcccagtt
      481 catctgcacc accggcgatg tgcccgtgcc ctggagcacc ctggtgacca ccctgaccgc
      541 aggcgcccag tgcttcgcca agtacggccc cgagctgaag gatttctaca agagctgcat
      601 gcccgatggc tacgtgcagg agcgcaccat caccttcgag ggtgatggca atttcaagac
      661 ccgcgccgag gtgaccttcg agaatggcag cgtgtacaat cgcgtgaagc tgaatggcca
      721 gggcttcaag aaggatggcc acgtgctggg caagaatctg gagttcaatt tcacccccca
      781 ctgcctgtac atctggggcg atcaggccaa tcacggcctg aagagcgcct
//
