LOCUS       sequence-66476-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM1000
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgaaaattga acaaacaaag ctcaccatct ggtcttccga
      181 aaagcaggtt gacatcctcc agaaactcgg ggaagaattc aaggcaaagt acggaatccc
      241 tgttgaagtt cagtacgtcg attttggaag catcaaatcc aaattcctga cggcggctcc
      301 acagggacag ggcgcagaca tcattgttgg agcgcacgac tgggtaggag aactcgccgt
      361 caacggtttg atcgaaccca ttcccaactt ctctgatctg aagaatttct atgacacggc
      421 tctcaaagct ttctcttacg gtggaaaact ctacggagtc ccgtacgcca tggaagcggt
      481 tgctctcatc tacaacaagg actacgttga ttctgttcct aagaccatgg acgagctcat
      541 agaaaaagca aaacagatag atgaggaata cggaggagaa gtcagaggtt tcatctacga
      601 tgtcgccaac ttctacttct ctgcgccgtt cattctaggt tacggaggat acgtcttcaa
      661 ggaaacacct cagggactcg acgtgacaga cattggactc gcgaacgaag gagcagtcaa
      721 aggtgcgaaa ctcataaaga gaatgatcga tgaaggtgtt ctcacccctg gtgacaacta
      781 cggaacgatg gattccatgt tc
//
