LOCUS       sequence-66480-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-TT (TetR, P21)
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    122..142
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      197..224
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(273..304)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    381..414
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 ttactagtag cggccgctgc aggcatgcct cgagatgcat ggcgcctaac ctaaactgac
      181 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tcgaacaatt
      241 gaaaaaacct cgcgccttac ctgttgagta atagtcaaaa gcctccggtc ggaggctttt
      301 gactttctgc ttactgaatt tcggtggtgc cgttaattaa ccggtgggcc ctcatgataa
      361 taatggtttc ttagacgtcc gaagttccta ttctctagaa agtataggaa cttccctagg
      421 tcagccaaac gtctcttcag gccactgact agcgataact ttccccacaa cggaacaact
      481 ctcattgcat gggatcattg ggtactgtgg gtttagtggt tgtaaaaaca cctgaccgct
      541 atccctgatc agtttcttga aggtaaactc atcaccccca agtctggcta tgcagaaatc
      601 acctggctca acagcctgct cagggtcaac gagaattaac attccgtcag gaaagcttgg
      661 cttggagcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga atgcagaatc
      721 actggctttt ttggttgtgc ttaccc
//
