LOCUS       sequence-66481-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE
  TITLE     One-step cloning and chromosomal integration of DNA.
  JOURNAL   ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. 
            Epub 2013 May 20.
  PUBMED    24050148
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400021j";
            journalName: "ACS Synth Biol"; date: "2013-09-20- 20"; volume: "2"; 
            issue: "9"; pages: "537-41"
COMMENT     Sequence Label: pOSIP-TT (TetR, P21)
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(27..58)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    135..168
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acctcgcgcc ttacctgttg agtaatagtc aaaagcctcc ggtcggaggc ttttgacttt
       61 ctgcttactg aatttcggtg gtgccgttaa ttaaccggtg ggccctcatg ataataatgg
      121 tttcttagac gtccgaagtt cctattctct agaaagtata ggaacttccc taggtcagcc
      181 aaacgtctct tcaggccact gactagcgat aactttcccc acaacggaac aactctcatt
      241 gcatgggatc attgggtact gtgggtttag tggttgtaaa aacacctgac cgctatccct
      301 gatcagtttc ttgaaggtaa actcatcacc cccaagtctg gctatgcaga aatcacctgg
      361 ctcaacagcc tgctcagggt caacgagaat taacattccg tcaggaaagc ttggcttgga
      421 gcctgttggt gcggtcatgg aattaccttc aacctcaagc cagaatgcag aatcactggc
      481 ttttttggtt gtgcttaccc atctctccgc atcacctttg gtaaaggttc taagcttagg
      541 tgagaacatc cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga
      601 cggctgcata ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa
      661 ttcttcaacg ctaactttga gaatttttg
//
