LOCUS       sequence-66483-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Gourisankar Ghosh unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET11a-p52(1-398)
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac atatggagag ttgctacaac ccaggtctgg atggtattat
      181 tgaatatgat gatttcaaat tgaactcctc cattgtggaa cccaaggagc cagccccaga
      241 aacagctgat ggcccctacc tggtgatcgt ggaacagcct aagcagagag gcttccgatt
      301 tcgatatggc tgtgaaggcc cctcccatgg aggactgccc ggtgcctcca gtgagaaggg
      361 ccgaaagacc tatcccactg tcaagatctg taactacgag ggaccagcca agatcgaggt
      421 ggacctggta acacacagtg acccacctcg tgctcatgcc cacagtctgg tgggcaagca
      481 atgctcggag ctggggatct gcgccgtttc tgtggggccc aaggacatga ctgcccaatt
      541 taacaacctg ggtgtcctgc atgtgactaa gaagaacatg atggggacta tgatacaaaa
      601 acttcagagg cagcggctcc gctctaggcc ccagggcctt acggaggccg agcagcggga
      661 gctggagcaa gaggccaaag aactgaagaa ggtgatggat ctgagtatag tgcggctgcg
      721 cttctctgcc ttccttagag ccagtgatgg ctccttctcc ctgcccctga agccagtcat
      781 ctcccagccc atccatgaca gcaaatctcc gggggcatca aacctgaaga tttctcgaat
      841 ggacaagaca gcaggctctg tgcggggtgg agatgaagtt tatctgctt
//
