LOCUS       sequence-66492-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Stellberger T, Hauser R, Baiker A, Pothineni VR, Haas J, Uetz P
  TITLE     Improving the yeast two-hybrid system with permutated fusions 
            proteins: the Varicella Zoster Virus interactome.
  JOURNAL   Proteome Sci. 2010 Feb 15;8:8. doi: 10.1186/1477-5956-8-8.
  PUBMED    20205919
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteome 
            Sci."; date: "2010-02-15"; volume: "8:8. doi"; pages: " 
            10.1186/1477-5956-8-8"
COMMENT     Sequence Label: pGBKCg
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    178..302
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(327..332,333..350,351..357)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(477..496)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaagtttgc tgtcttgcta tcaagtataa atagacctgc aattattaat cttttgtttc
       61 ctcgtcattg ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct
      121 ataccaagca tacaatcaac tccaagcttg aagcaagcct cctgaggtac cgcatcaaca
      181 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      241 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      301 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      361 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      421 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      481 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      541 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      601 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      661 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      721 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      781 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      841 t
//
