LOCUS       sequence-66507-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-ACO2
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2..18)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3..19)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(432..451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(433..451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(461..478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(461..477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(705..724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgataccgt cgacctcgag aacacaaccc acagctacca ccatcaacaa tatttatata
       61 tataacgtac acatagaaat cacacaaaca gagtatttat tcttaactac atgaactacc
      121 atcagaccgt ctgggccact atataatgtg ccattcataa acgtgatcac tttacgtagc
      181 aggcaacccc aggtgaaaat ttttcagcga gctgccagat tgtcaggtga aaaactgaaa
      241 aaaacttctg ggcgatgagc ttgtggcggg aaattaagta tataaagcag ttagtttctt
      301 ctgcttcttg tggttctggg tattcttgta atatttggaa gaatagaaaa tcaagactac
      361 tgcctttctt ttcatattat agaggaagaa atacgcacga acacgatata gaggtaaagg
      421 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      481 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      541 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      901 c
//
