LOCUS       sequence-66517-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-MET3
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(8..24)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(438..457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(439..457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(467..484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(467..483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(476..498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agcttatcga taccgtcgac ctcgagaaca caacccacag ctaccaccat caacaatatt
       61 tatatatata acgtacacat agaaatcaca caaacagagt atttattctt aactacatga
      121 actaccatca gaccgtctgg gccactatat aatgtgccat tcataaacgt gatcacttta
      181 cgtagcaggc aaccccaggt gaaaattttt cagcgagctg ccagattgtc aggtgaaaaa
      241 ctgaaaaaaa cttctgggcg atgagcttgt ggcgggaaat taagtatata aagcagttag
      301 tttcttctgc ttcttgtggt tctgggtatt cttgtaatat ttggaagaat agaaaatcaa
      361 gactactgcc tttcttttca tattatagag gaagaaatac gcacgaacac gatatagagg
      421 taaaggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      481 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      541 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      601 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      841 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttt
//
