LOCUS       sequence-66518-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pGRB2.0
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..124
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     33..49
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     34..50
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(84..100)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(84..100)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(137..157)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(137..155)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(176..192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(189..211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    200..216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(224..230,231..248,249..254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    269..290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(407..424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(658..677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actatagggc gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga
       61 tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga
      121 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
      181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc aga
//
