LOCUS       sequence-66520-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pGRB2.2
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     596..612
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     596..612
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(646..662)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(647..663)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccataaa gcacgtggcc
       61 tcttatcgag aaagaaatta ccgtcgctcg tgatttgttt gcaaaaagaa caaaactgaa
      121 aaaacccaga cacgctcgac ttcctgtctt cctattgatt gcagcttcca atttcgtcac
      181 acaacaaggt cctagcgacg gctcacaggt tttgtaacaa gcaatcgaag gttctggaat
      241 ggcgggaaag ggtttagtac cacatgctat gatgcccact gtgatctcca gagcaaagtt
      301 cgttcgatcg tactgttact ctctcccttt caaacagaat tgtccgaatc gtgtgacaac
      361 aacagcctgt tctcacacac tcttttcttc taaccaaggg ggtggtttag tttagtagaa
      421 cctcgtgaaa cttacattta catatatata aacttgcata aattggtcaa tgcaagaaat
      481 acatatttgg tcttttctaa ttcgtagttt ttcaagttct tagatgcttt ctttttctct
      541 tttttacaga tcatcaagga agtaattatc tactttttac aacaaatata aaacatctag
      601 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
      661 cgagaacaca acccacagct accaccatca acaatattta tatatataac gtacacatag
      721 aaatcacaca aacagagtat ttattcttaa ctacatgaac taccatcaga ccgtctgggc
      781 cactatataa tgtgccattc ataaacgtga tcactttacg tagcaggcaa ccccaggtga
      841 aaatttttca gcgagctgcc agattgtcag gtgaaaaact gaaaaaaact tctgggcgat
      901 gagcttgtgg cggg
//
