LOCUS       sequence-66521-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pGRB2.3
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..83)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(67..83)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(653..673)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(653..671)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(705..727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    716..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(740..746,747..764,765..770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    785..806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgaa ttcctgcagc
       61 ccgggggatc cactagttct agatgtttta tatttgttgt aaaaagtaga taattacttc
      121 cttgatgatc tgtaaaaaag agaaaaagaa agcatctaag aacttgaaaa actacgaatt
      181 agaaaagacc aaatatgtat ttcttgcatt gaccaattta tgcaagttta tatatatgta
      241 aatgtaagtt tcacgaggtt ctactaaact aaaccacccc cttggttaga agaaaagagt
      301 gtgtgagaac aggctgttgt tgtcacacga ttcggacaat tctgtttgaa agggagagag
      361 taacagtacg atcgaacgaa ctttgctctg gagatcacag tgggcatcat agcatgtggt
      421 actaaaccct ttcccgccat tccagaacct tcgattgctt gttacaaaac ctgtgagccg
      481 tcgctaggac cttgttgtgt gacgaaattg gaagctgcaa tcaataggaa gacaggaagt
      541 cgagcgtgtc tgggtttttt cagttttgtt ctttttgcaa acaaatcacg agcgacggta
      601 atttctttct cgataagagg ccacgtgctt tatggagctc cagcttttgt tccctttagt
      661 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      841 gaaacctgtc gtgccagctg cattaatgaa
//
