LOCUS       sequence-66528-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-DnvLB16 M40R;W41H;H42S
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(353..382)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(827..853)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(854..865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attgtaatac gactcactat agggcgaatt ggagctcaat tctcttagga ttcgattcac
       61 attcatcttt ttttagctac taataggata aattatagga atttataaca aattgaaata
      121 tggcaggcag caaaattaaa attgtcttag ttttttagtg tatagaagtg aatagctata
      181 taaagtatgt gtaaagttgg taacggaacg aaaaatagaa aaggatatta catgggaaaa
      241 catgttgttt acggagaaat gaaaagtata ttgtattttg tacgagctaa aagtacagtg
      301 ggaacaaagt cgattttgtt acatctacac tgttgttatc agatctctat tacaagtcct
      361 cttcagaaat aagcttttgt tcggatccgc ccccctcgag tttgtaacct atcaaccagt
      421 caggcttttc aggcaaggag atttcagtac cactcaatgt gtaatattcc ttcaatactt
      481 ccaagaattc ttcccacaaa acgtccaaag cttccttcca ttgttcgaaa tcttccttgg
      541 cgtagaatgt ccagtttgca gaaccagcaa ttttgtcagc cttgaatttg aagtgcattg
      601 gcaaaactct caacaagttg ttaccagcca aagagtgcct agtttggata atgaattcga
      661 tttcatcttc taaggcggtg tagtaaacgt aagaggaaaa ggcttcaatc aaagggatgt
      721 aaaaggccca caatctccat tcgatggcta ataatctcat atggctagcc gaccctccgc
      781 ctccgctacc gcctccacca gagcctcctc caccactagc ctgcagagcg tagtctggaa
      841 cgtcgtatgg gtatctacct tcaatcgtcg agctattgtc cttaaaa
//
