LOCUS       sequence-66529-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-MET3-GFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(200..415)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(439..458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     558..580
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(618..636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
       61 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
      121 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
      181 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
      241 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
      301 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
      361 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt
      421 ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      601 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      661 aatggtttct tagcaggtta gactaacatg caaaaagttt atattcattt ttgtagttat
      721 tcttgttttc attaggatga ttacacactg tggatgtgga ttaatcgatc attgttaatt
      781 tagtgtagct actaccattt caaacaaaac gtaaattctt gcagtcgtac tggatctgtg
      841 aatctattag tatatatgaa ttaaagtagc ttgtacatat
//
