LOCUS       sequence-66530-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-DnvLB16 M40R;W41H;H42S
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..124
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             456..467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             468..494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
       61 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
      121 cgtcaaggag aaaaaacccc ggatcgaatt ccctacttca tacattttca attaagatgc
      181 agttacttcg ctgtttttca atattttctg ttattgcttc agttttagca caggaactga
      241 caactatatg cgagcaaatc ccctcaccaa ctttagaatc gacgccgtac tctttgtcaa
      301 cgactactat tttggccaac gggaaggcaa tgcaaggagt ttttgaatat tacaaatcag
      361 taacgtttgt cagtaattgc ggttctcacc cctcaacaac tagcaaaggc agccccataa
      421 acacacagta tgtttttaag gacaatagct cgacgattga aggtagatac ccatacgacg
      481 ttccagacta cgctctgcag gctagtggtg gaggaggctc tggtggaggc ggtagcggag
      541 gcggagggtc ggctagccat atgagattat tagccatcga atggagattg tgggcctttt
      601 acatcccttt gattgaagcc ttttcctctt acgtttacta caccgcctta gaagatgaaa
      661 tcgaattcat tatccaaact aggcactctt tggctggtaa caacttgttg agagttttgc
      721 caatgcactt caaattcaag gctgacaaaa ttgctggttc tgcaaactgg acattctacg
      781 ccaaggaaga tttcgaacaa tggaaggaag ctttggacgt tttgtgggaa gaattcttgg
      841 aagtattgaa ggaatattac acattgagtg gtactgaaat ctcct
//
