LOCUS       sequence-66531-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCN-MET3-GFP
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..573)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARELGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 ttaggggcag ggcatgctca tgtagagcgc ctgctcgccg tccgaggcgg tgccgtcgta
       61 cagggcggtg tccaggccgc agagggtgaa ccccatccgc cggtacgcgt ggatcgccgg
      121 tgcgttgacg ttggtgacct ccagccagag gtgcccggcg cccagctcgc gggcgaactc
      181 cgtcgcgagc cccatcaacg cgcgcccgac cccgtgcccc cggtgctccg gggcgacctc
      241 gatgtcctcg acggtcagcc ggcggttcca gccggagtac gagacgacca cgaagcccgc
      301 caggtcgccg tcgtccccgt acgcgacgaa cgtccgggag tcagggtcgc cgtcctcccc
      361 gtcgtccgat tcgtcgtccg attcgtcgtc ggggaacacc ttggtcaggg gcgggtccac
      421 cggcacctcc cgcagggtga agccgtcccc ggtggcggtg acgcggaaga cggtgtcggt
      481 ggtgaaggac ccatccagtg cctcgatggc ctcggcgtcc cccgggacac tggtgcggta
      541 tcggtaagcc gtgtcgtcaa gagtggtgcc cattttagat ccaaaactta gattagattg
      601 ctatgctttc tttctaatga gcaagaagta aaaaaagttg taatagaaca agaaaaatga
      661 aactgaaact tgagaaattg aagaccgttt attaacttaa atatcaatgg gaggtcatcg
      721 aaagagaaaa aaatcaaaaa aaaaattttc aagaaaaaga aacgtgataa aaatttttat
      781 tgcctttttc gacgaagaaa aagaaacgag gcggtctctt ttttcttttc caaaccttta
      841 gtacgggtaa ttaacgacac c
//
