LOCUS       sequence-66533-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pET29b-HtsptLB12v.3
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             645..662
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(715..733)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      729..776
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata tacatatggg ttccgaaatg agtgaaatgt
      181 ctgttgtaga ttacgtcgtt aagtctttta attatacaga ggatatgagt catgaattgt
      241 tattctctgt taaaaagaga tggttcgtaa agccattcga acactcaacc agatatggtt
      301 tgttagccta catgttgtta cctggtaact acataaagtt cggtttgtac gttttaacga
      361 accaagacta tgctagattc gaaatagcat gggtacatgt cgataaagac ggtaaaatcg
      421 aagaaagaac cgtatattcc attgaaactt actggcacat tttcattgat atcgaaaacg
      481 acttgaactg tccatacgtt ttggcaaagt ttattgaaat gagacctgaa ttcgataaga
      541 cagcctgggt cgaagaatca aactattcca ttgctgaaga tgacatccaa atggttgaaa
      601 gtataaaaag atacttggaa agaaagatcg cttctgatct cgagcaccac caccaccacc
      661 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      721 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      781 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      841 ggcgggtgtg gtggttacgc gcagcgtgac cgctaccttg ccagcgccta gcgccgctct
      901 tcgcttctcc ttct
//
