LOCUS       sequence-66537-e        1884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP tagged Tau in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Hoover BR, Reed MN, Su J, Penrod RD, Kotilinek LA, Grant MK, 
            Pitstick R, Carlson GA, Lanier LM, Yuan LL, Ashe KH, Liao D
  TITLE     Tau mislocalization to dendritic spines mediates synaptic 
            dysfunction independently of neurodegeneration.
  JOURNAL   Neuron. 2010 Dec 22;68(6):1067-81. doi: 
            10.1016/j.neuron.2010.11.030.
  PUBMED    21172610
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2010.11"; journalName: "Neuron"; date: 
            "2010-12-22- 22"; volume: "68"; issue: "6"; pages: "1067-81"
COMMENT     Sequence Label: pRK5-EGFP-Tau
FEATURES             Location/Qualifiers
     source          1..1884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13..15,16..18,19..729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSNQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(58..79)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(319..338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     666..687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      481 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag caaccagtcc gccctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      721 ctgtacaagg gatccgctga gccccgccag gagttcgaag tgatggaaga tcacgctggg
      781 acgtacgggt tgggggacag gaaagatcag gggggctaca ccatgcacca agaccaagag
      841 ggtgacacgg acgctggcct gaaagctgaa gaagcaggca ttggagacac ccccagcctg
      901 gaagacgaag ctgctggtca cgtgacccaa gctcgcatgg tcagtaaaag caaagacggg
      961 actggaagcg atgacaaaaa agccaagggg gctgatggta aaacgaagat cgccacaccg
     1021 cggggagcag cccctccagg ccagaagggc caggccaacg ccaccaggat tccagcaaaa
     1081 accccgcccg ctccaaagac accacccagc tctggtgaac ctccaaaatc aggggatcgc
     1141 agcggctaca gcagccccgg ctccccaggc actcccggca gccgctcccg caccccgtcc
     1201 cttccaaccc cacccacccg ggagcccaag aaggtggcag tggtccgtac tccacccaag
     1261 tcgccgtctt ccgccaagag ccgcctgcag acagcccccg tgcccatgcc agacctgaag
     1321 aatgtcaagt ccaagatcgg ctccactgag aacctgaagc accagccggg aggcgggaag
     1381 gtgcagataa ttaataagaa gctggatctt agcaacgtcc agtccaagtg tggctcaaag
     1441 gataatatca aacacgtccc gggaggcggc agtgtgcaaa tagtctacaa accagttgac
     1501 ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca tccatcataa accaggaggt
     1561 ggccaggtgg aagtaaaatc tgagaagctt gacttcaagg acagagtcca gtcgaagatt
     1621 gggtccctgg acaatatcac ccacgtccct ggcggaggaa ataaaaagat tgaaacccac
     1681 aagctgacct tccgcgagaa cgccaaagcc aagacagacc acggggcgga gatcgtgtac
     1741 aagtcgccag tggtgtctgg ggacacgtct ccacggcatc tcagcaatgt ctcctccacc
     1801 ggcagcatcg acatggtaga ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc
     1861 tccctggcca agcagggttt gtga
//
