LOCUS       sequence-66538-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-EGD2-GFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..83)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(67..83)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(446..466)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(446..464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(485..501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(485..501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(498..520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    509..525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(533..539,540..557,558..563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    578..599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(716..733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgaa ttcctgcagc
       61 ccgggggatc cactagttct agactttgta tatctgtatt attgacgtgt ttttgatttg
      121 tccctattgc aacagctact caattgacca aaattgtgta gcaataacaa cttatatgtg
      181 ctatgagacc ctccaaagct cttcgatgaa agaaattttg aaaaaaaaaa gcgtcgacga
      241 gaaatgtagc gttgtacgtg tttcttgcca cgggggtaat gtacgtacag cattaccata
      301 ataagctcat catcatcttt cagtttgagt atcccatata tttggagtgg gttcggccat
      361 ctcggtagcc acagcgtata taagggatgg tgactgggca acttattact ggtgagtgaa
      421 gtggacagag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
      481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      661 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      721 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      781 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaa
//
