LOCUS       sequence-66539-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCN-EGD2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(8..24)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9..25)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(461..477)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(461..477)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(474..496)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    485..501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(509..515,516..533,534..539))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    554..575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(692..709)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcttatcga taccgtcgac ctcgagaaca caacccacag ctaccaccat caacaatatt
       61 tatatatata acgtacacat agaaatcaca caaacagagt atttattctt aactacatga
      121 actaccatca gaccgtctgg gccactatat aatgtgccat tcataaacgt gatcacttta
      181 cgtagcaggc aaccccaggt gaaaattttt cagcgagctg ccagattgtc aggtgaaaaa
      241 ctgaaaaaaa cttctgggcg atgagcttgt ggcgggaaat taagtatata aagcagttag
      301 tttcttctgc ttcttgtggt tctgggtatt cttgtaatat ttggaagaat agaaaatcaa
      361 gactactgcc tttcttttca tattatagag gaagaaatac gcacgaacac gatatagagg
      421 taaaggtacc ttgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      481 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      541 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      601 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      661 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac c
//
