LOCUS       sequence-66547-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Neo DEST (w531-1)
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    96..220
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(245..250,251..268,269..275)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(395..414)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggatcca ccggttcgtc
       61 gactagtcca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagctgaac
      121 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      181 ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc
      241 aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc
      301 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      361 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      421 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      481 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      541 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      601 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      661 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      721 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      781 gagtttcacc agttttgatt taaacgtggc caatatggac aacttct
//
