LOCUS       sequence-66549-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, PGK 
            promoter, Neo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti PGK Neo DEST (w531-1)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..193
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(234..539)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(336..355)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgagcggccg ccactgtgct
       61 ggatatcaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca
      121 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      181 tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
      241 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
      301 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
      361 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
      421 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
      481 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
      541 tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc
      601 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
      661 ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
      721 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
      781 tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg
      841 catactgtta tctggctttt agtaagccgg atccagatct ttacgccccg ccctgcc
//
