LOCUS       sequence-66556-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative selection marker for use with the Mos-1 mediated system of 
            creating targeted transgene insertions and targeted deletions in C. 
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pMA122 - peel-1 negative selection
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    518..542
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctttgtatag aaaagttgct acaggtcgac tctagaggat caccaaaaac ggaacgttga
       61 gctggacgga aatagtggta aagtgacatg attatagttt gaagatttct aatttcacaa
      121 ttagagcaaa tgttgttcgg tatttatttt caacggtatt tatactattt tccacctttt
      181 tctagaacat tcgagctgct tgttgcaaaa ggagggcgac tcacattcgg tacatggaaa
      241 agtagtgtac acaataaaga gacccagata cattttccgt ctgcgtctct ttgcacccac
      301 cgggagtatt ttcaaacgaa tgcatctagg accttctaga acattctgta aggctgcaga
      361 atgcgggtat ataaggaaag cgggctcaga ggaagccaac acgctttgtt ctagtgcatc
      421 taaaaaactt cgaaaatcct catcggatcc tatcgattcg cggccgctgt acacccgggt
      481 gccctatagt gagtcgtatt ggtaccggta gaaaaaatca agtttgtaca aaaaagcagg
      541 cttcatgcgc tttgatttcc aaaacttaaa attttcgatg attttcatat ttttgtggaa
      601 catcatagtc ggatttttac ttgctcttgt aaagattttc aaaatctaca tgtatcttga
      661 tctgcctgaa aatgtatggg taaatcggtt tgcgcatgtt attgctctta ttgga
//
