LOCUS       sequence-66558-e         629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mos1 transposase expression vector for use in creating targeted 
            transgene insertions and targeted deletions in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ601 - Peft-3 Mos1 transposase
FEATURES             Location/Qualifiers
     source          1..629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(92..116)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      121..444
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(448..468)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(476..493)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(476..492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(485..507)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaagacgatg agttctactg gcgtggaatc cacaaattgc ccgagagatg ggaaaaatgt
       61 gtcgcgagcg acggcaaata ctttgaatag tacccagctt tcttgtacaa agtgggataa
      121 atgcaaaatc ctttcaagca ttcccttctt ctctatcact cttctttctt tttgtcaaaa
      181 aattctctcg ctaatttatt tgctttttta atgttattat tttatgactt tttatagtca
      241 ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg tctgatgtac
      301 tttcacaatc tctcttcaat tccattttga agtgctttaa acccgaaagg ttgagaaaaa
      361 tgcgagcgct caaatatttg tattgtgttc gttgagtgac ccaacaaaaa gaggaaactt
      421 tattgtgccg ccaagaaaaa agtctcacaa ctttattata catagttgat aattcactgg
      481 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      541 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      601 cccaacagtt gcgcagcctg aatggcgaa
//
