LOCUS       sequence-66564-e         331 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Zeng M, Kuzirian MS, Harper L, Paradis S, Nakayama T, Lau NC
  TITLE     Organic small hairpin RNAs (OshR): A do-it-yourself platform for 
            transgene-based gene silencing.
  JOURNAL   Methods. 2013 May 23. pii: S1046-2023(13)00148-5. doi: 
            10.1016/j.ymeth.2013.05.007.
  PUBMED    23707624
REFERENCE   2  (bases 1 to 331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2013 May 23. pii: S1046-2023(13)00148-5. doi: 
            10.1016/j.ymeth.2013.05.007."
COMMENT     Sequence Label: pGSH0
FEATURES             Location/Qualifiers
     source          1..331
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           50..92
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(163..191)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtttagggaa ccgccattct gcctggggac gtcggagcaa gctagcttgc ttgttctttt
       61 tgcagaagct cagaataaac gctcaacttt ggcagatctg aattcctgca gcccggggga
      121 tccacagcca ccatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggtacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tggccaacac ttgtcactac tctcacttat g
//
