LOCUS       sequence-66569-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantFLAG-puro-kana
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(89..122)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             168..188
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             195..218
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(236..269)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(904..921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctaacta attgagatgc
       61 atgctttgca tacttctgcc tgctggaaat aacttcgtat agcatacatt atacgaacgg
      121 tactcgaggg gaagcttgcc gccgatatca ccagcctgta caagggcgag aatttgtatt
      181 ttcagggtga gctcgattac aaggatgacg acgataagta gactagtgga tcccctaccg
      241 ttcgtatagc atacattata cgaagttatt gatcaaccgg actgttacca acacccacac
      301 ccctgtgatg aaacaaaacc cataaatgcg cataaaacaa gacgagattg gcatggcttt
      361 atttgttttt tcttttggcg cttgactcag gattaaaaaa ctggaatggt gaaggtgtca
      421 gcagcagtct taaaatgaaa catgttggag cgaacgcccc caaagttcta caatgcatct
      481 gaggactttg attgtacatt tgtttctttt ttaatagtca ttccaaatat tgttataatg
      541 cattgttaca ggaagttact cgcctctgtg aaggcaacag cccagctggg aggagccggt
      601 accaattact ggtgttagat gataattgct tgtctgtaaa ttatgtaacc caacaagtgt
      661 ctttttgtat cttccgcctt aaaaacaaaa cacacttgat cctttttggt ttgtcaagca
      721 agcgggctgt gttccccagt gatagatgtg aatgaaggct ttacagtccc ccacagtcta
      781 ggagtaaagt gccagtatgt gggggaggga ggggctacct gtacactgac ttaagaccag
      841 ttcaaataaa agtgcacaca atagaggctt gactggtgtt cccagggggc gctcttccgc
      901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg c
//
