LOCUS       sequence-66574-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantFLAG-bsr-kana
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(443..463)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          593..658
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatctctagt caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa
      181 agctaaaggt acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg
      241 gagtaagaaa aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag
      301 aatatttttc cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag
      361 caaagcaagc aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg
      421 aaggaaagtc cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag
      481 agtcagcagt agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct
      541 cattaaaggc attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata
      601 cacaaacaat tagaatcagt agtttaacac attatacact taaaaatttt atatttacct
      661 tagagcttta aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt
      721 ccttcacaaa gatccggacc aaagcggcca tcgtgcctcc ccactcctgc agttcggggg
      781 catggatgcg cggatagccg ctgctggttt cctggatgcc gacggatttg cactgccggt
      841 agaactccgc gaggtcgtcc agcctcaggc agcagccaag ctagcttggt atggtaaaac
      901 ttttaatttc gggtatattt gagtggaatg agttcttcaa tcgtagtttt gactaacttg
      961 ccattcattt ctattaacac aaaacaatct ggtgcatagt c
//
