LOCUS       sequence-66579-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantA-bsr-kana
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(442..462)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          592..657
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
       61 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      121 atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
      181 gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
      241 agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
      301 atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
      361 aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
      421 aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
      481 gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
      541 attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
      601 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
      661 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
      721 cttcacaaag atccggacca aagcggccat cgtgcctccc cactcctgca gttcgggggc
      781 atggatgcgc ggatagccgc tgctggtttc ctggatgccg acggatttgc
//
