LOCUS       sequence-66583-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: TroponinT-GCaMP5-Zeo
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(66..87)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(300..356)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(384..398,399..407))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(411..443)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(447..464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(474..498)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(529..548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(530..548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accatgctcc ctccggtacc gcccttgtac agctcgtcca tgccgagagt gatcccggcg
       61 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttcgaaagt
      121 ttggactgca cgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      181 gtgttctgct ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      241 ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcct tgatatagac gttctcgagt
      301 gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt acgacgtgac
      361 gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg acccatttgc
      421 tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc catagcctgc
      481 ttttttgtac aaacttgttg atcaagctta cctagccagc ttgggtctcc ctatagtgag
      541 tcgtattact cgagggtggc ggatcctgga ggcgtctgct cagtctcagc ggggactggg
      601 tgaggcagag gatggagagg gctttaagca ggcatgtggg ctggggcctg gtgagccagc
      661 cctgcggagg gaggaatgtg cgacaggg
//
