LOCUS       sequence-66595-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantA-puro-kana
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(89..122)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             168..188
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             228..401
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             402..575
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     protein_bind    complement(623..656)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctaacta attgagatgc
       61 atgctttgca tacttctgcc tgctggaaat aacttcgtat agcatacatt atacgaacgg
      121 tactcgaggg gaagcttgcc gccgatatca ccagcctgta caagggcgag aatttgtatt
      181 ttcagggtga gctcaaaacc gcggctcttg cgcaacacga tgaagccgtg gacaacaaat
      241 tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag
      301 aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt
      361 tagcggaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca
      421 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac
      481 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag
      541 aagctaaaaa gctaaatggt gctcaggcgc cgaaagtaga cgcgaattcc gcggggaagt
      601 caacctgaac tagtggatcc cctaccgttc gtatagcata cattatacga agttattgat
      661 caaccggact gttaccaaca cccacacccc tgtgatgaaa caaaacccat aaatgcgcat
      721 aaaacaagac gagattggca tggctttatt tgt
//
