LOCUS       sequence-66596-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantEGFP-bsr-kana
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          789..854
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gaagtagtgg ggaggctttt ttggaggcct aggcttttgc aaaaagctag cttgcatgcc
       61 tgcaggtcgg ccgccacgac cggtggattc atttttgcag aaatcggagg aagaagaata
      121 tatgaaaaca tttaacattt ctcaacaaga tctagaatta gtagaagtag cgacagagaa
      181 gattacaatg ctttatgagg ataataaaca tcatgtggga gcggcaattc gtacgaaaac
      241 aggagaaatc atttcggcag tacatattga agcgtatata ggacgagtaa ctgtttgtgc
      301 agaagccatt gcgattggta gtgcagtttc gaatggacaa aaggattttg acacgattgt
      361 agctgttaga cacccttatt ctgacgaagt agatagaagt attcgagtgg taagtccttg
      421 tggtatgtgt agggagttga tttcagacta tgcaccagat tgttttgtgt taatagaaat
      481 gaatggcaag ttagtcaaaa ctacgattga agaactcatt ccactcaaat atacccgaaa
      541 ttaaaagttt taccatacca agctagcttg gctgctgcct gaggctggac gacctcgcgg
      601 agttctaccg gcagtgcaaa tccgtcggca tccaggaaac cagcagcggc tatccgcgca
      661 tccatgcccc cgaactgcag gagtggggag gcacgatggc cgctttggtc cggatctttg
      721 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
      781 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      841 tttgtgtatt ttagattcca
//
