LOCUS       sequence-66597-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, 
            Dziembowski A
  TITLE     A new strategy for gene targeting and functional proteomics using 
            the DT40 cell line.
  JOURNAL   Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. 
            Epub 2013 Jul 27.
  PUBMED    23892402
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2013-09"; volume: "41(17):e167. doi"; pages: " 
            10.1093/nar/gkt650. Epub 2013 Jul 27"
COMMENT     Sequence Label: pQuantEGFP-bsr-kana
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(438..458)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          588..653
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      121 ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat taaaaagcta
      181 aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct gtgtggagta
      241 agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt tacagaatat
      301 ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta aatagcaaag
      361 caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa ttctgaagga
      421 aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg ttgagagtca
      481 gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt ttcctcatta
      541 aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
      601 acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
      661 ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
      721 acaaagatcc ggaccaaagc ggccatcgtg cctccccact cctgcagttc gggggcatgg
      781 atgcgcggat agccgctgct ggtttcctgg atgccgacgg atttgcactg ccggtagaac
      841 tccgcgaggt cgtccagcct caggcagcag cca
//
