LOCUS       sequence-66605-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     src plasmids
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV5 mouse src K295R Y527F
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(8..521)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(203..224)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     415..434
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    567..791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     780..797
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggtacccacc ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa
       61 tatttaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      121 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      181 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      241 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      301 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      361 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      421 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      481 caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatccc ccctcgagcc
      541 ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag catgcctgct
      601 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
      661 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
      721 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
      781 agaaggcaca gtcgaggctg atca
//
