LOCUS       sequence-66629-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Monnat RJ Jr, Hackmann AF, Cantrell MA
  TITLE     Generation of highly site-specific DNA double-strand breaks in human
            cells by the homing endonucleases I-PpoI and I-CreI.
  JOURNAL   Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93.
  PUBMED    10082660
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "1999-02-5"; volume: "255(1)"; pages: 
            "88-93"
COMMENT     Sequence Label: pCNPpo6
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          154..250
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             320..340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
      121 actcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
      181 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
      241 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
      301 cggccaattc accatggctc caaaaaaaaa gcgcaaagtg gcgctcacca atgctcaaat
      361 cttggctgtg attgacagct gggaagaaac agtcggtcag tttccagtga taacgcacca
      421 tgtaccatta ggtggcggtc tgcaaggaac gctccattgt tacgagatcc ccctagcagc
      481 tccttatggg gttggctttg ctaagaatgg gcctacccgc tggcaataca aacggacaat
      541 caatcaagtc gtccacagat ggggatccca cacagtccct tttctattag aaccggataa
      601 catcaacggc aaaacctgca cagcatcgca cctatgtcat aatactcgat gccacaatcc
      661 cttgcacttg tgctgggagt cactagacga caacaaaggc agaaactggt gcccgggtcc
      721 caacggggga tgtgtccatg cggtggtttg tttaaggcag ggtccgttgt acggcccggg
      781 ggcgactgtg gcaggtcctc aacaaagggg cagtcacttt gtggtataag gtctgaggtt
      841 actagatccg ggtaccgagc tcgaat
//
