LOCUS       sequence-66641-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Monnat RJ Jr, Hackmann AF, Cantrell MA
  TITLE     Generation of highly site-specific DNA double-strand breaks in human
            cells by the homing endonucleases I-PpoI and I-CreI.
  JOURNAL   Biochem Biophys Res Commun. 1999 Feb 5;255(1):88-93.
  PUBMED    10082660
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun."; date: "1999-02-5"; volume: "255(1)"; pages: 
            "88-93"
COMMENT     Sequence Label: pCNCre3
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          154..250
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             320..340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
      121 actcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
      181 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
      241 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
      301 cggccaattc accatggctc caaaaaaaaa gcgcaaagtg gcgctgaata caaaatataa
      361 taaagagttc ttactctact tagcagggtt tgtagacggt gacggtagca taatcgctca
      421 aattaagcct aatcagtctt ataaatttaa gcatcagcta tcactcacgt tccaagtcac
      481 gcaaaagaca cagagacgtt ggtttttagg caaattagtg gatgaaattg gggttggtta
      541 tgtaagagat aggggtagcg tttcggatta tattctaagc gaaatcaagc ctttgcataa
      601 ttttttaaca caactacaac cttttctaaa actaaaacaa aaacaagcaa atttagtttt
      661 aaaaattatt gaacaacttc cgtcagcaaa agaatccccg gacaaattct tagaagtttg
      721 tacatgggtg gatcaaattg cagctctgaa tgattcgaag acgcgtaaaa caacttctga
      781 aaccgttcgt gctgtgctag acagtttaag tgaaaaaaag aaatcgtc
//
