LOCUS       sequence-66642-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo recombination assay fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Li H, Ulge UY, Hovde BT, Doyle LA, Monnat RJ Jr
  TITLE     Comprehensive homing endonuclease target site specificity profiling 
            reveals evolutionary constraints and enables genome engineering 
            applications.
  JOURNAL   Nucleic Acids Res. 2012 Mar;40(6):2587-98. doi: 10.1093/nar/gkr1072.
            Epub 2011 Nov 25.
  PUBMED    22121229
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkr1072"; journalName: "Nucleic Acids Res"; date: 
            "2012-03"; volume: "40"; issue: "6"; pages: "2587-98"
COMMENT     Sequence Label: pDR-GFPuniv
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             59..79
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      293..302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(344..365)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(605..624)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tatgggatca agatcgccaa aaaagaagag aaaggtgccg aagaagcatg cagcaccacc
       61 aaaaaaaaaa cgaaaagtag aagacccacg aggcaacacc agcggcgtgc tgagcacccc
      121 caaggccaag agggccaagc acccccccgg caccgagaag cccaggagca ggagccagag
      181 cgagcagccc gccacctgcc ccatctgcta cgccgtgatc aggcagagca ggaacctgag
      241 gaggcacctg gagctgaggc acttcgccaa gcccggcgtg gatccaccgg tcgccaccat
      301 ggtgagcaag ggcgaggggc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      361 cgacgtaaac ggccacaagt tcagcgtgtc cggcctcgag ggtaccgagc tcacctacgg
      421 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      481 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      541 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      601 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      661 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      721 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      781 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      841 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      901 c
//
