LOCUS       sequence-66643-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo recombination assay fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Li H, Ulge UY, Hovde BT, Doyle LA, Monnat RJ Jr
  TITLE     Comprehensive homing endonuclease target site specificity profiling 
            reveals evolutionary constraints and enables genome engineering 
            applications.
  JOURNAL   Nucleic Acids Res. 2012 Mar;40(6):2587-98. doi: 10.1093/nar/gkr1072.
            Epub 2011 Nov 25.
  PUBMED    22121229
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkr1072"; journalName: "Nucleic Acids Res"; date: 
            "2012-03"; volume: "40"; issue: "6"; pages: "2587-98"
COMMENT     Sequence Label: pDR-GFPuniv
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(56..514,515..517,518..520))
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     195..214
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     454..475
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      517..526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(740..760)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(782..802)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catgattacg ccaagctttt attgaagttc acctgatgcc gttcttctgc ttgtcggcca
       61 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      121 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      181 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      241 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      301 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      361 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      421 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      481 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatcca
      541 cgccgggctt ggcgaagtgc ctcagctcca ggtgcctcct caggttcctg ctctgcctga
      601 tcacggcgta gcagatgggg caggtggcgg gctgctcgct ctggctcctg ctcctgggct
      661 tctcggtgcc gggggggtgc ttggccctct tggccttggg ggtgctcagc acgccgctgg
      721 tgttgcctcg tgggtcttct acttttcgtt ttttttttgg tggtgctgca tgcttcttcg
      781 gcacctttct cttctttttt ggcgatcttg atccctaaag cttgggctgc aggtcgaggg
      841 atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
      901 tggctagtaa aacatgtaag gaaaatttta gggatgtt
//
