LOCUS       sequence-66650-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: TroponinT-GCaMP5-Zeo
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(574..627)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcagc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gtctagtcct gttcctcagc tacaaaatgg acacaatttc cagcagggtc
      241 tctgagggca aattcccttc cccaaggttg ttcaccaatt tctgtcatgg ctgggccaga
      301 ggcatccctg aaatttgtgc tgactacttc tgaccattct gcataaagct catctaggcc
      361 tctgacccag acccaagcaa gggtgttgtc agggacaact tggtcctgaa ctgctgagat
      421 gaagagggtg acatcatctc tgacaacacc agcaaaatca tcttcaacaa agtctctgga
      481 gaatcctaat ctgtcagtcc agaactctac agcccctgca acatcccttg ctgtgaggac
      541 tgggactgca gaagtgagtt tggccatacc ggaagggccg ggattctcct ccacgtcacc
      601 gcatgttaga agacttcctc tgccctctcc ggatcccttc gctgtcatca tttgtacaaa
      661 ctcttcgtag tttacctgac cgtccccatc gatgtctgct tccctgatca tttcatcaac
      721 ctcttcatct gttaacttct ctccaaggtt tgtcatcacg tggcgaagct ctgctgcact
      781 gatgtagcca ttgccatcct tatcaaacac acggaacgct tctctaattt cttcttcact
      841 gtctgtgtat ttcatttttc
//
