LOCUS       sequence-66654-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-MEF2C
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcagc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gcccatgagg cctgatgttg cccatccttc agaaagtcgc atgcgcttga
      241 ctgagggact ttccctttcg tccggcgaag gtctggtgag tccaatgggg gagtggaatt
      301 cgttccggtg atcctctcgg tcgctcccgt cgtacgaact gctacagctg ctcaagctgt
      361 caacaggaga tctccccgcc tcgtggcgcg tgtgttgtgg gtatctcgaa ggggtggtgg
      421 tacggtctct aggaggagaa acaggttctg acttgatgtt gaggctttga gtagaaggca
      481 gggagagatt tgaactctga gataaatgag tgctagtgca agctcccaac tgactgaggg
      541 cagatggtgg catgttatgt aggtgttgct gttgccagcc agttactgaa ccaaggtgaa
      601 gagcgctggc ggtgttaaac ccagacagag atgacaggtc tgcactactc agagagtact
      661 cggtaccata tgttgttgaa atggctgatg gatatcctcc cattccttgt cctggtaaag
      721 taggagttgc tacggaaacc actgggg
//
