LOCUS       sequence-66655-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-TBX5
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcagc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gtccaagcta ttgtcgctcc actctggcac catgccaact ccgtgcacag
      241 agtggtactg atgaggggat agagtccttg gcacgccatg agagtagagg aactcagggg
      301 gctgaagggt gccaggggac tgcaggccag tctgaggccc acactgcctg accacaggct
      361 ggtgggccac ggaggtctgg tgctggaaca ttccctctcc cagctgtggg gagccatggt
      421 tggccatgcc agccagccga gggaccaggg gccccgaggt gaagtgagcg gagaagtgct
      481 ggtagggtag cctgtccatg ggctgcacgg tggtgacggt gcagctgctg taggaaggca
      541 tgcttggcca cgtgttgcag ctgatgtcct ctaggctggg cacaggctcg ctggggggcg
      601 cagagctggc atacatgcaa gcttgccgct gtgccgactc tgtcctgtag gaggcaccca
      661 ggccctgctg ctgtggatag ctagagcggt agaaggaatc ttcttcactg ggtgatgtct
      721 ccatgtaggg cttcttatag ggatggtctg tggtggaaca ttcttcctct ttcctcttgg
      781 tacaatggta aatttggcta tgctcctggg gca
//
