LOCUS       sequence-66657-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-Myocd
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcagc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gcccatgagg ccctaccact gctgtaagtg gagatccata ggggaattca
      241 gattaagatc tgtaacatcc agaaaatcga tgttgaaaat gctgggccca ctggaggtaa
      301 ggttgctgaa gcctggcgtg gaactaggtg gagtgaggtc cagccattcc attgtttccc
      361 aaggagattc cgtgaagctc agctgcagac cactgctgtc cacagaagga ggtgacaact
      421 gtgcatgcat cggggagagg ggcccaggca agagctcgtg agcactgaag agatcttccg
      481 cagccttccc tgggaagcca tccatgatgc tgtcaaaagg aggctcctcg cttccgattt
      541 tgaggagggt aacatcgctc acctttccga tggggctgtg agaattcaat aagacttcca
      601 ggtgttcgtc actgtcgttg gcgtagtgat cgaaggccat ctggcctccc gaagatgcct
      661 gctcaaagga agccgagggc ttcgggggga tggcagttgg gctgcaggag gacccaggga
      721 tctttggaat tttctgaaga catgaatgat cttccctggc atcggctggc atttctccac
      781 tttcaatgag gacatccagg agttcgtcca tctgctgact ccgagtcatt tgctgcttca
      841 ctgcatcttc ataggatggg ggctgggtga tatctgaaac tgctgaactt gactta
//
