LOCUS       sequence-66660-e        7014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA-CAD with S1900 mutated to alanine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ben-Sahra I, Howell JJ, Asara JM, Manning BD
  TITLE     Stimulation of de novo pyrimidine synthesis by growth signaling 
            through mTOR and S6K1.
  JOURNAL   Science. 2013 Mar 15;339(6125):1323-8. doi: 10.1126/science.1228792.
            Epub 2013 Feb 21.
  PUBMED    23429703
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1228792"; journalName: "Science"; date: 
            "2013-03-15- 15"; volume: "339"; issue: "6125"; pages: "1323-8"
COMMENT     Sequence Label: pcDNA3-FLAG-HA-CAD S1900A
FEATURES             Location/Qualifiers
     source          1..7014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             92..115
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tcgatatcgc cggccgccat ggactacaag gacgacgatg acaagtaccc
      121 ttatgacgtg cccgattacg ctagcggccg ctcgtctgcg tgcttgccct gtctcagctc
      181 cgacccgatg gcggccctgg tgttggagga cgggtcggtg ctgcagggcc ggccctttgg
      241 ggccgctgtg tcgactgctg gggaagtggt gtttcagacc ggcatggtcg gctacccaga
      301 ggccctcact gacccttcct acaaagctca gatcctagtg ctaacgtatc ctctcatcgg
      361 caactacggc attccctcag atgaagagga cgagttcggc ctgagcaagt ggtttgaatc
      421 ctcggagatc cacgtggcgg gacttgtagt gggagaatgc tgtcccacac ccagccactg
      481 gagtgccaac tgcaccctgc atgagtggct gcaacagcgt ggcatccccg gcctgcaagg
      541 agtggacact cgggagctga ctaagaagtt gcgggagcaa gggtctcttt tggggaagct
      601 ggtccagaaa gggacagagc cgtcagccct gccattcgtg gaccccaatg ccaggcccct
      661 ggcaccagag gtctctatta agactccacg ggtattcaat gcagggggtg cccctcggat
      721 ctgtgctctg gattgtggcc tcaagtataa tcagatccga tgtctctgcc agcttggggc
      781 tgaagttacc gtggtaccct gggaccacga gttagacagt cagaaatatg acggtctctt
      841 cctaagtaat ggacccggtg accctgcctc ttacccgggt gtggtgtcca cactgagccg
      901 agtcttgtct gaacctaacc cccgacctgt gtttggaatc tgccttggac accagctgtt
      961 ggctttagcc atcggggcca aaacttacaa gatgagatac ggaaaccgag gccacaacca
     1021 gccctgttta ctggtgggca ctgggcgctg ctttctgacg tcccagaatc atgggtttgc
     1081 agtggacgca gattcgctgc cagcaggctg ggctcctctg ttcaccaatg ccaatgactg
     1141 ttccaatgaa ggcatagtgc acgacagcct gcccttcttc agtgtccagt ttcacccaga
     1201 gcaccgggcc ggcccttcag atatggaact gctcttcgat gtatttctag aaactgtgag
     1261 agaggctgca gctgggaaca ttgggggcca gacagtaaga gaacgcttgg ctcagcgcct
     1321 ctgtccccct gagctcccca ttcctggttc tgggctcccg cctccacgaa aggttctaat
     1381 cctaggctca gggggcctct ccattggcca agctggagaa tttgactact cgggctctca
     1441 ggcaattaaa gccctaaagg aggaaaacat ccagacatta ctaatcaacc ccaacattgc
     1501 cacagtgcag acttctcagg ggctggcaga caaggtctat ttccttccca taacacttca
     1561 ctacgtaacc caggtaattc gtaatgaacg cccagatggt gtgttactga cttttggggg
     1621 tcaaacagcc ctgaactgcg gtgtggagct gaccaaagct ggggtactgg ctcgatatgg
     1681 ggtccgggtc ttgggtacac ccgtggagac cattgaactg actgaggacc gacgagcctt
     1741 cgctgccagg atggctgaga tcggagagca tgtcgccccc agtgaagcgg caaattctct
     1801 tgaacaggct caggcagctg ccgagcgact aggctaccct gtgctggtgc gagcagcctt
     1861 tgccctgggt ggtctcggtt ctggctttgc ttccaccaaa gaggaactct cagctcttgt
     1921 ggctccagct tttgcccata ccagtcaggt gctgatagac aagtctctga agggctggaa
     1981 ggagattgaa tatgaggtgg tgagagacgc ctatggcaac tgcgtaacgg tatgcaacat
     2041 ggagaactta gacccactgg gcattcatac tggtgaatcc atagtggtgg ctccgagcca
     2101 gacactgaat gacagagagt accagcttct gcgacggaca gctatcaagg tcactcagca
     2161 cctgggcatt gtcggggagt gcaatgtaca gtatgctttg aaccccgagt ctgagcagta
     2221 ttacatcatt gaagtgaatg ccaggctctc ccgaagctct gccctggcca gtaaggccac
     2281 aggctatcca ctagcctatg tggcagccaa gctagcattg ggcattcccc tgccagagct
     2341 cagaaactct gttacgggag gaacagcagc ctttgagccc agcctggact actgcgtggt
     2401 aaagattcct cgctgggacc tcagcaagtt cctacgtgtc agtacgaaga ttgggagttg
     2461 catgaagagt gttggcgagg tcatgggcat cgggcggtcc tttgaggagg ccttccaaaa
     2521 ggccctgcgc atggtcgatg agaactgcgt aggcttcgac catacagtaa agccagtcag
     2581 cgacatggag ctggagactc cgacggataa gcggatcttt gtggtggctg ccgctctgtg
     2641 ggctggctac tctgtggagc gcctctacga gctcacccgc attgattgct ggttcctgca
     2701 ccggatgaag cgcattgtga cccacgccca gttgctggaa caacaccgtg gacaagcttt
     2761 accccaagac ctgctgcacc aggccaagtg cctcggcttc tcagacaagc agattgccct
     2821 ggcagtcctg agcacagagc tggctgttcg gaagctgcgt caggaactgg ggatctgccc
     2881 agcggtgaaa cagattgaca cagttgcagc tgagtggcca gcccagacaa attacctgta
     2941 cctgacatac tggggcaaca cccatgacct cgactttcgg gcacctcacg tcctggtcct
     3001 tggctctggt gtctaccgga ttggctccag tgttgagttt gattggtgtg ctgtgggttg
     3061 catccagcag ctccggaaga tgggttataa gaccatcatg gtgaactaca acccagagac
     3121 agtcagcacc gactatgata tgtgtgaccg actctacttt gatgagatct cttttgaggt
     3181 ggtgatggac atctatgagt tagagaaccc cgaaggtgtg atcctgtcca tgggtgggca
     3241 gctgcccaac aacatggcca tggctttgca tcggcagcag tgccgagtgc tgggcacctc
     3301 ccccgaagcc atcgactcag ccgagaaccg attcaagttc tcccggctcc tagataccat
     3361 cggtatcagc cagcctcagt ggcgagagct cagtgacctt gagtctgctc gccagttctg
     3421 ccacacggtc gggtacccct gcgtagtgcg cccctcctat gtgctcagcg gtgctgctat
     3481 gaatgtggcg tacaccgacg gggacctgga gcgtttcctg agcagtgcgg ccgccgtctc
     3541 taaggagcac cccgtagtca tctccaagtt cattcaggaa gcaaaggaga tcgacgtgga
     3601 cgcggtggcc tgtgacggca tcgtgtcggc cattgccatc tctgagcacg tggagaatgc
     3661 aggtgtgcat tcaggtgatg ccacgctggt gaccccccca caagacatca ccccgaaaac
     3721 tctggagcgg atcaaagcca ttgtgcatgc tgtgggccag gagctacagg ttacaggacc
     3781 cttcaatctg cagctcattg ccaaggatga ccagctgaag gttattgaat gcaacgtgcg
     3841 tgtatctcgc tctttcccct tcgtgtccaa gacactgggt gttgatctag tagccttggc
     3901 cacaaggatc atcatggggg agaaagtaga gcccgtgggg ctcatgactg gctctggagt
     3961 cgtgggagta aaggtacctc agttctcctt ctcacgcttg gccggtgccg acgtggtact
     4021 tggtgtggag atgaccagta ctggagaggt ggccggcttc ggagagagcc gttgtgaagc
     4081 ctacctcaaa gccatgctca gcactggctt taagatccca gagaagaaca tcctgctgac
     4141 tattggcagc tacaagaaca aaagtgagct gctcccaact gtgcggttgc tggagagcct
     4201 gggctacagc ctctatgcca gcctgggtac ggcagacttc tacactgagc acggggtcaa
     4261 ggtaacagct gtggactggc actttgaaga ggcggtggat ggtgagtgcc caccacaacg
     4321 gagcatcttg gatcagctgg ctgaaaatca ctttgagcta gtgattaacc tgtccatgcg
     4381 tggggctggg ggtcggcgtc tttcctcctt cgttaccaag ggctaccgta cccggcgtct
     4441 ggccgctgat ttctctgtgc ctctcatcat tgacatcaag tgcaccaaac tctttgtgga
     4501 ggccctaggc cagatcggcc cagcccctcc tttgaaagtg cacgttgact gcatgacctc
     4561 ccagaagcta gtgcgcctgc ccggattgat tgatgtccac gtgcaccttc gggaaccagg
     4621 tgggacacac aaagaggact ttgcctcagg cacagctgct gccttggctg ggggtgtcac
     4681 catggtttgt gctatgccta atacccgacc ccccatcatt gatgctcctg ctctcgccct
     4741 tgcccagaag ctggcagagg ctggcgcccg ctgtgacttt accttattcc ttggagcctc
     4801 atctgagaac gcggggactc tgggtgctgt ggccggctct gcagcaggcc tgaagctcta
     4861 cctcaacgag accttctctg agctgcggct ggacagtgtg gcccagtgga tggagcattt
     4921 tgaaacctgg cctgcccacc tccccattgt ggcccatgca gagcggcaga gtgtcgctgc
     4981 ggtcctcatg gtggctcagc tgacccagcg tccagtgcac atatgtcatg tggctcggaa
     5041 ggaagagatc ctgttgatta aaactgcaaa ggcgcagggg ctgccagtga cctgtgaggt
     5101 cgctcctcac cacctgttcc taaaccggga agacttggag cgcctgggac ctgggaaggg
     5161 agaggtccgt cctgagcttg ggtcccgaga ggatatggaa gccctgtggg agaatatggc
     5221 agtcatcgac tgttttgcct cagaccacgc tccccatacc ttggaagaga agtgtgggcc
     5281 caagcctcca cctggcttcc cgggcctaga gaccatgttg ccgctgctgc tgacagctgt
     5341 gagcgagggc cgcctcagtc tggatgacct gctgcagcga ttgcaccaca accctcggcg
     5401 gatcttccac ctgcctctcc aggaggacac ctatgtagag gtggatctgg agcatgagtg
     5461 gacagtcccc agccacatgc ccttctctaa ggcccgctgg accccatttg aaggacagaa
     5521 ggtgaagggc acagtgcgcc gtgtggtctt gcgaggggag gtcgcctata ttgatggaca
     5581 ggtgctggta cccccgggct atggacaaga tgtacggaag tggccgcagg gggttgttcc
     5641 ccagccccct ccttcaactc ccgccaccac cgaaatcacc acgacacctg agcgaccacg
     5701 ccgagtcatc ccgggccttc ctgatggccg cttccacctg ccaccccgca tccaccgggc
     5761 ctctgaccca ggtctgccag ctgaggaacc taaagagaag ccacccagga aggtagtgga
     5821 accagagctg atgggaaccc ctgatggtcc ctgctaccct gcaccaccag tacctagaca
     5881 ggcagcaccc cagaacctag ggtcttctgg cctgctgcac ccccagatgt cgcccctact
     5941 gcactcccta gtgggccagc acatcctgtc tgtcaagcag ttcactaagg atcagatgtc
     6001 tcatctgttc aatgtggcgc acacgctacg gatgatggta cagaaggagc ggagcctcga
     6061 catcctgaag gggaaggtca tggcctccat gttctacgag gtgagcaccc gcaccagtag
     6121 ctccttcgca gcagccatgg cccggctcgg gggcgctgtt ctcagcttct cagaagctac
     6181 atcctccgtc caaaagggcg agtcccttgc tgactcggtg cagaccatga gttgctatgc
     6241 tgatgtcatt gtgctccgac accctcagcc tggagccgtg gagctggcag ccaagcactg
     6301 tcgcaggcca gtgatcaatg ctggggatgg agttggggag caccccacac aggccctgct
     6361 ggacatcttc accattcgag aagagctggg gactgtcaat ggcatgacga tcactatggt
     6421 cggtgacctg aagcatggac gcacagtgca ctccctggcc tgcctgctca cccagtaccg
     6481 tgtgagccta cgctacgtgg cacctcccag cctgcgcatg ccacccagtg tacgggactt
     6541 tgtggcctcc cgaggcacca agcaggagga gtttgagagc atcgaggagg cgctgcccga
     6601 cacagacgtg ctctacatga ctcggatcca gaaagaacga tttggctctg tccaggaata
     6661 tgaagcttgc tttggtcagt tcatcctcac tcctcacatc atgacccggg ccaagaagaa
     6721 gatggtggtg atgcacccaa tgccccgtgt caatgagata agtgtggagg tggactcgga
     6781 cccccgggca gcctacttcc gccaagctga gaacggcatg tacatccgca tggccctgct
     6841 tgccaccgtg cttggccgtt tctaggtccc agcttccttt cttgtctctt caggcccagc
     6901 tgctgggcaa ggaatcccga tgcccccccc acgggggcag cacacttagc agacaatcct
     6961 ggagcataca gatagcccaa gcacatagat gtactaaact gggaccatgc ttta
//
