LOCUS       sequence-66664-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pKR2.40
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(158..282)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(377..397)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
      181 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      241 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatctag ttctagagcg
      301 gccgccaccg gtggagctcc accgatcccc cgggcatttg tatagttcat ccatgccatg
      361 tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct tttcgttggg
      421 atctttcgaa agggcagatt gtgtggacag gtaatggttg tctggtaaaa ggacagggcc
      481 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc catcttcaat
      541 gttgtgtcta attttgaagt tctgaaaatt taaatcagat tagttagtta gtaaaatcat
      601 gtttaaactt acaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      661 tgagttatag ttgtattcca atttgtgtcc a
//
