LOCUS       sequence-66673-e         494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Swanson C, Saintigny Y, Emond MJ, Monnat RJ Jr
  TITLE     The Werner syndrome protein has separable recombination and survival
            functions.
  JOURNAL   DNA Repair (Amst). 2004 May 4;3(5):475-82.
  PUBMED    15084309
REFERENCE   2  (bases 1 to 494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Repair 
            (Amst)."; date: "2004-05-4"; volume: "3(5)"; pages: "475-82"
COMMENT     Sequence Label: pMM288
FEATURES             Location/Qualifiers
     source          1..494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    159..177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    243..261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             416..445
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             455..484
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac
       61 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
      121 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      181 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      241 tttctctatc actgataggg agtggtaaac tcgacctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      361 gaccgatcca gcctccgcgg ccccgaattn ctgcaggaat cgatttaaag ctatggagca
      421 aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt ctgaagagga
      481 cttgaatgaa atgg
//
