LOCUS       sequence-66689-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: tetO-GATA4
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..91)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(101..142)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    168..192
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattgag gctgatcagc
       61 gggtttaaac tcaatggtga tggtgatgat gaccggtacg cgtagaatcg agaccgagga
      121 gagggttagg gataggctta ccttcgaacc gcgggccctc tagatcaacc actttgtaca
      181 agaaagctgg gttcgcagtg attatgtccc cgtgactgtc ggccaagacc aggctgttcc
      241 aggagtcctg cttggagctg gtctgtggag actggctgac gggagacgca tagccttgtg
      301 gggagagctt cagggccgag aggacagggt ggatggaggg cccatggcca gacatcgcac
      361 tgactgagaa cgtctgggac acggagctgc tgtgcccgta gtgagatgac aggccaggct
      421 ccgtcttgat gggacgcatc tcctcgctgc tgctggtggt ggcgttgctg gagttgctgg
      481 aagcaccgct ggcgggagga aggctctcac tgcctgaagg agctgctggt gtcttagatt
      541 tattcaggtt cttgggcttc cgttttctgg tttggatccc ctctttccgc attgcaagag
      601 gcctggggac cccgtggagc ttcatgta
//
