LOCUS       sequence-66710-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Shb-SH2
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..22
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             356..382
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(441..463)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(608..626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggatctggtt ccgcgtggat cccctgccgt ccccctggag aagcaaatat ggtatcacgg
       61 agccatcagc agaggagacg ccgagaacct gctgcgactc tgcaaggagt gtagctacct
      121 tgtccggaac agccagacca gcaagcatga ctactccctc tccctgagga gcaaccaggg
      181 ttttatgcac atgaaactgg ccaaaaccaa agagaaatac gttctgggtc agaacagccc
      241 tccgttcgac agtgtcccgg aagtcatcca ctactacacc accagaaagc tacccatcaa
      301 aggggctgag cacttgtccc tcctctatcc cgtggctgtg aggacccggg aattctatcc
      361 ctatgatgtt cccgattatg ccgaattcat cgtgactgac tgacgatctg cctcgcgcgt
      421 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      481 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      541 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga
      601 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      661 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      721 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      781 tattgaaaaa gga
//
