LOCUS       sequence-66719-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX Vav1-SH2
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             408..452
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     primer_bind     complement(516..538)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(683..701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgttccaggg gcccctggga tccggccctc ctcaggacct gtctgttcat ctctggtacg
       61 caggccccat ggagcgggca ggggcagaga gcatcctggc caaccgctcg gacgggactt
      121 tcttggtgcg gcagagggtg aaggatgcag cagaatttgc catcagcatt aaatataacg
      181 tcgaggtcaa gcacattaaa atcatgacag cagaaggact gtaccggatc acagagaaaa
      241 aggctttccg ggggcttacg gagctggtgg agttttacca gcagaactct ctaaaggatt
      301 gcttcaagtc tctggacacc accttgcagt tccccttcaa ggagcctgaa aagagaacca
      361 tcagcaggcc agcaggaatt catcgtgacg caagcttgct tggtggcggt ctgaacgaca
      421 tcttcgaggc tcagaaaatc gaatggcacg aataagcggc cgcatcgtga ctgactgacg
      481 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      601 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
      661 tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      721 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      781 atttgtttat ttttctaaat acattcaaat atgtatccgc t
//
